植物生長素性物質に関する研究-11-

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The investigation on the plant growth-promoting substances in the bamboo shoot (Phyllostachys edulis) were carried out, applying paper chromatography(2, 3, 4, 5, 6, 7) as bioassay method. In this method the squares cut from the chromatograms of the ether extracts of bamboo shoot, were extracted with water, and each of them was tested on the extension of Avena coleoptile sections. Each of the acid, non-acid, and hydrolysed acid fractions were extracted with ether, and the free amino acid fraction with warm water, by the modified extraction methods of YAMAKI(8) and BONDE(9) (cf. Table 1). It may be concluded from the application of bioassay method of paper chromatography that the growth-promoting substances in the bamboo shoot were localized in the zones of RF 0.0_??_0.1, 0.35_??_0.5 and 0.7_??_0.85, of which the last RF value was found to coincide almost perfectely with that of synthetic indole-3-acetic acid (cf. Figure 1). As to the acid fractions in the top, middle and bottom of the shoot, the coleoptile extension was most conspicuous in the top (cf. Figure 2). Tryptophan, supposed to be a precursor of indole-3-acetic acid, could not be identified in the free amino acid fraction, but sixteen amino acids-aspartic acid, glutamic acid, serine, asparagine, glycine, threonine, glutamine, lysine, alanine, tyrosine, arginine, γ-amino butyric acid, methionine, valine, leucine, phenylalanine were found by means of paper chromatography (cf. Figure 3).
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植物生長素性物質に関する研究-11-

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