ランの無菌発芽培養基に関する研究-2-播種密度および培養期間の違いと,培地の陽イオンおよび陰イオン処理がシランの実生の生育に及ぼす影響〔英文〕

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The relationships between anionic and cationic combinations relevant to seed population and culture period of Bletilla striata seedlings were shown. Total concentration of the final nutrient media was held to 20 milligram equivalents per liter.1. The germination was not much affected by ionic treatments.2. The growth of seedlings on a medium was affected by population density. For higher seeding populations, the effect of the medium was significant for the first 10 weeks. That is; the fresh weight and roots per plant were lower. Whereas for lower seeding populations plants were relatively small.3. The growth of seedlings in ionic treatments differed with age, and anionic ratios concentrated the optimum at a point.4. The requirement for anionic balances was relatively accurate and seedlings grew well at high nitrate levels, whereas that for cationic balances was not clarified. The optimal balances of ions expressed as a percentage of the total ionic concentration in the chemical equivalent were determined as follows; NO3-: H2PO4-: SO4--=60:30:10, and NH4+:K+:Ca++:Mg++=20:40:30:10.5. One of the optimal nutrient compositions for the Bletilla striata seedling growth is as follows; NH4NO3 0.320g/l MgSO4•7H2O 0.247g/l KH2PO4 0.817 Sucrose 20 KNO3 0.202 Agar 10 Ca(NO3)2•4H2O 0.945
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