ファレノプシス類の微細繁殖法の改善

概要

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Micropropagation of Phalaenopsis through flower stalk culture was investigated. Bracts of flower stalks were removed before or after sterilization. Decontamination rate of nodal sections was higher when they were sterilized with bract. Subsequent growth of lateral buds was affected by the timing of bract removal. Much lateral bud developed flower stalk secondary when bracts were removed after sterilization, but much of them developed vegetative shoots when bract was removed before sterilization. Propagation from a plantlet was investigated using in vitro clone plantlets. Base part of a plantlet, 1.5 cm was cut into 2.5 mm or 5 mm slice segments, and they were cultured on NP medium with or without coconut water (CW) and/or 6-benzylaminopurine (BA). Regeneration of shoot(s) and callus like body (CLB) was dominant in the slice segments derived from 5-10 mm part from the base of the shoot and was promoted by addition of CW and/or BA.
2000-03-01