Lipid analysis of the biopsy specimen of the liver

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概要

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• Lipid analysis of the biopsy specimen of the human liver was carried out in 6 cases of nonspecific hepatitis, 11 cases of chronic hepatitis, 10 cases of liver cirrhosis, 5 cases of the fatty liver and in 5 cases of mild non-hepatic disease.Biopsy was performed under laparoscopic visual field and the diagnosis was made on the histological findings. Lipids were extracted with chloroform-methanol 2:1, separated on 1D and 2D TLC and estimated by colorimetric assay of phosphorus, ester-linkage and cholesterol.Lipid composition of the control liver in non-hepatic disease showed the total phospholipid 2.95% (±0.29) of the wet weight of the liver, free cholesterol 0.17%, ester cholesterol 0.19%, and triglyceride 0.20-0.80%.In cases of non-specific reactive hepatitis, ester cholesterol was slightly decreased, while. the other components did not show a marked change. In chronic hepatitis and in the early stage of liver cirrhosis, accumulation of triglyceride was observed. Ester cholesterol was also increased in these cases. The liver showing the triglyceride content more than 1% of the wet weight of the tissue was found to have a fatty change on histological observation. Total phospholipid was significantly decreased in most of the cases of chronic hepatitis and liver cirrhosis (2.40±0.22% in chronic hepatitis and 1.4-2.2% in liver cirrhosis). Cholesterol was also decreased. Phosphorus analysis on 2D TLC showed that phosphatidyl ethanolamine was decreased in most of the cases of chronic hepatitis and liver cirrhosis.In cases of hyperlipemia, the decrease of total phospholipid was not so marked as in the early stage of the liver cirrhosis which showed the same extent of the fattt change. In all of the cases of hyperlipemia and in a case of progressive lipodystrophy, an acidic phospholipid tentatively identified as lyso-bis-phosphatidic acid was increased.In a case of hyperlipemia which showed a storage of lipoid substance in the liver, lymphnodes and the bone marrow, this phospholipid encounted 21% of the total phospholipid.Microdetermination of liver lipids in the biopsy specimen seems to be important in the study on fatty liver and hyperlipemia.

• 財団法人 日本消化器病学会の論文

著者

• 山本 章

  大阪大学医学部木谷内科

• 石部 武

  大阪大学医学部第2内科

• 足立 進

  大阪大学医学部

• 足立 進

  大阪大学医学部第2内科

• 礒崎 正弘

  大阪大学医学部第2内科

• 垣内 義亨

  大阪大学医学部第2内科

• 進土 義剛

  大阪大学医学部第2内科

• 山本 章

  大阪大学医学部

• 山本 章

  大阪大学医学部第2内科

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