Phagocytic activity of rat Kupffer cells. The measurement method in vitro and the effect of hepatocytes upon the phagocytic activity.:The measurement method in vitro and the effect of hepatocytes upon the phagocytic activity

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The measurement method of the phagocytic activity of Kupffer cells (=KC) was investigated and the effect of hepatocytes (=HC) upon the phagocytic activity of KC was studied in vitro.Phagocytic activity of KC was measured by the uptake rate of 59Fe by KC (2×106) incubated with chondroitin sulfate 59Fe for 24 hours (=phagocytic activity rate: PAR). When KC were co-cultured with HC (2×105, 5×105, 1×106 cells) for 24 hours, the PAR increased in proportion to the increase in number of HC (4.13, 5.37, 6.28%), comparing with that of KC alone (3.36%). And when KC were cultured with the supernate of HC (5×105) which were cultured for 24 hours, this PAR (4.15%) was superior to that of KC alone, but didn't exceed that of KC co-cultured with HC (5×105).The PAR was remarkably suppressed by the inhibitor of glycolysis (NaF), but was not supressed so much by the inhibitor of oxidative phosphorylation (NaN3).The measurement method of phagocytic activity of KC in vitro was established, and it was proved that the PAR was enhanced by co-cultured HC and the supernate of clutured HC, and depended on glycolytic metabolism.
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