マリ-ゴ-ルドカルス中の殺線虫活性とα-タ-チオフェン含量〔英文〕

概要

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Tissue cultures of marigold (Tagetes patula L.) were established and a high nematocidal activity was detected in n-hexane extracts from the calli. The nematocidal activity varied with callus cell lines that were established on Murashige and Skoog (MS) basal medium supplmented with different concentrations of auxin and cytokinin. The highest nematocidal activity was found in the green solid callus on the medium with 0.1ppm 1-naphthaleneacetic acid. The nematocidal activity and the level of α-terthiophene in the green calli were equivalent to those in the root of intact plants cultivated in vitro. HPLC analysis of n-hexane extracts from several callus cell lines indicated that the nematocidal activity was due predominantly to the level of α-terthiophene but other nematocidal components are also present.
日本植物細胞分子生物学会の論文