:A new method of continuous time course observation

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Everted intestinal sacs have widely been utilized for in vitro studies of absorption of various nutrients. The ordinary everted sac method, however, can give only one point's value. We have developed a new method which enabled us to observe the continuous time course of glucose absorption by an everted intestine. In this method the upper end of a sac was tied over a small polyethylene sampling tip and an aliquot (10μl) was collected from the serosal side every 12 minutes. The same amount of fluid was also collected from the mucosal side at the same time interval. The collected fluids were analyzed for glucose concentration by using a Beckman Autoanalyzer.Using this method, we tested glucose absorption of 8 week-old female Sprague-Dawley rats. We found that glucose absorption of both upper and middle portions of the small intestine was remarkable as evidenced by an increase in glucose concentration in the serosal fluid. The serosal glucose concentration reached a maximum at about 84min. after the start of incubation, and the high concentration was sustained until 180 minutes. On the other hand the glucose absorption by the lower portion of the ileum was very little, e.i. the glucose concentration in the serosal side increased only slightly or insignificantly.
財団法人日本消化器病学会の論文