Amine decomposition by enterobacteria
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概要
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Experiments on amine production by intestinal bacteria have been reported in the previous papers. In this study, decomposition of various amines produced from amino acid by intestinal bacteria was examined.Amines used in this experiments were histamine, agmatine, putrescine and tyramine. Intestinal bacteria were isolated from feces of healthy persons. Bacteria were cultured in liquid culture medium containing each amine. And then decomposition substances in culture filtrate were examined by various chromatographical techniques. Amine oxidase activity was determined by Warburg's manometric method.Amine oxidase activity was higher in E. coli and Klebsiella in general, L. bifidus and L. acid. had not so much for this activity. Various bacteria had considerable difference on amine decomposition. Some strains had some amine oxidase activities or not so. Various bacteria tested in this study did not produce N-acetyl tyramine from tyramine, but p-hydroxyphenylacetic acid was proved. As the decomposition product of histamine, imidazol acetic acid was proved. As that of agmatine and putrescine, α-amino butyric acid was proved.Amine production and decomposition in the intestinal canal would be affected by amino acid decarboxylase and amine oxidase of enterobacteria. And then it was demonstrated that optimun pH of these enzymes had considerable differences.It was clarified that amine concentration in the digestive canal was affected by many factors in digestive canal.
- 財団法人 日本消化器病学会の論文
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