Microdetermination of Adrenocortical Steroids by Double Isotope Method (III) Distribution and Incorporation of Corticosteroids into Haman Blood cell Fractions
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概要
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By using the double isotope derivative dilution method, in which the carbonyl group at C-3 alone is derived to thiosemicarbazone-<SUP>35</SUP>S as reported previously, microdetermination of cortisol, corticosterone, cortisone, aldosterone, deoxycorticosterone, and progesterone in erythrocytes, granulocytes, lymphocytes, and platelets of human adult male blood was carried out. The relationship between the quantity of these corticosteroids added and their incorporation into these cell fractions, and the saturation volume of incorporation and the relationship between saturation volume and steroid structure were examined.<BR>In normal human blood, corticosteroids amounting to 38.46-58.85% of the total quantity are present in plasma, those amounting to 26.10-36.55% in erythrocytes, 3.02-8.55% in granulocytes, 2.50-4.27% in lymphocytes, 0.72-2.30% in platelets, and 8.74-11.58% in other fractions. The quantity of corticosteroids calculated as the amount per cell in granulocytes and lymphocytes was about 150-300 times larger than that in erythrocytes, and 0.7-1.6 times lager than that of erythrocytes even in platelets.<BR>Incorporation of corticosteroids into blood cell fractions <I>in vitro</I> differed markedly with the kind of steroids and cell. The saturation volume in blood cell fractions was about 3-10 times larger in steroids with small polarity than in those with large polarity. This fact proves that the saturation volume of incorporation of corticosteroids in blood cell fractions is correlated the polarity of steroids. Furthermore, the saturation volume calculated per cell in granulocyte, lymphocyte, and platelet was about 25-130, 16-50, and 1-4 times that in erythrocyte, respectively.
- 社団法人 日本内分泌学会の論文
著者
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MATSUMOTO Ushiho
Department of Analytical Chemistry, Tokyo College of Pharmacy
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Kunugi Akira
Department Of Chemical Science And Technology Institute Of Science And Technology The University Of
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NAGASE YUZO
Department of Analytical Chemistry, Tokyo College of Pharmacy
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Matsumoto Ushiho
Department Of Analytical Chemistry Tokyo College Of Pharmacy
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Kunugi Akira
Department Of Chemical Science And Technology Faculty Of Engineering The University Of Tokushima
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KUNUGI AKIRA
Department of Analytical Chemistry, Tokyo College of Pharmacy
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