Growth Hormone Releasing Factor Activity in the Stalk-Median Eminence and Plasma Growth Hormone Response to the Ether-Laparotomy Stress in the Rat

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概要

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• The present study was performed to aim to the development of the <I>in vitro</I> assay method for growth hormone releasing factor (GH-RF) activity in the rat stalk-median eminence (SME), and to reveal the relationship between GH-RF activity in SME and plasma GH levels under ether-laparotomy stress in the rat.<BR>SME extract was incubated with a total of 8 quartered anterior pituitaries. GH released into the medium was measured by polyacrylamide gel disc electrophoresisdensitometry system, and was used as an index of GH-RF activity. Plasma GH levels were estimated by the Rat GH radioimmunoassay kit (NIAMD).<BR>Under manual handling, no significant change occurred in the levels of GH-RF activity in SME and in plasma GH levels. The ether-laparotomy (EL) stress caused a significant rise in GH-RF activity in SME at 15min and reciprocally a significant decrease in plasma GH at 5min and 15min after starting of EL stress. Namely, there was an inverse relationship between the response of GH-RF activity in SME and that of plasma GH under EL stress. These findings may suggest that the EL stress depress the GH secretion <I>via</I> the hypothalamus, and diminition of GH secretion could be attributed to inhibition of GH-RF release or increased secretion of an inhibitory factor.

• 社団法人 日本内分泌学会の論文

著者

• OGAWA NORIO

  Third Department of Internal Medicine, Okayama University Medical School

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