Routine Ultramicro-Measurement of Human Growth Hormone in Plasma by Solid-Phase Radioimmunoassay
スポンサーリンク
概要
- 論文の詳細を見る
A method for the ultramicro-measurement of human growth hormone (HGH) in plasma based on the solid-phase radioimmunoassay (RIA) using antibody-coated disposable microtiter trays was described. This method is simple, rapid and inexpensive, and particularly useful for ultramicro-measurement of plasma HGH levels in large numbers of plasma samples.<BR>The lower limit of sensitivity for purified HGH was 25pg/m<I>l</I> (P<0.05). The precision of the measurement of HGH in plasma has been calculated, and the quantitative sensitivity of the method derived from the results given by its routine use was 56.8pg/m<I>l</I> (P<0.05). The HGH level of normal pooled plasma in 10 replicates was 1, 515pg/m<I>l</I> (±126;1SD), and the coefficient of variation was 8.3%.The mean basal plasma HGH level after an overnight fasting in 23 normal males was 2, 100pg/m<I>l</I> (±1, 491; 1 SD) by this method and 2, 036pg/m<I>l</I> (1, 643; 1 SD) by double-antibody RIA, and in 23 normal females, 2, 589pg/m<I>l</I> (±1, 189; 1 SD) and 3, 300 pg/m<I>l</I> (±1, 690; 1 SD) respectively; there was no statistically significant difference between the basal HGH level obtained from this method and that from double-antibody RIA in normal subjects. On the other hand, the mean basal plasma HGH level after an overnight fasting in 21 hypopituitary patients was 484pg/m<I>l</I> (±282; 1 SD) by this method, and this value was significantly lower (P<0.001) than 1, 376pg/m<I>l</I> (±498; 1 SD) by double-antibody RIA. These data show that determination of basal plasma HGH levels by this method is of much value in the diagnosis of hypopituitarism.
- 社団法人 日本内分泌学会の論文
著者
関連論文
- IMMUNOHISTOCHEMICAL DISTRIBUTION OF NEUROPEPTIDES IN THE RAT CENTRAL NERVOUS SYSTEM
- ULTRASTRUCTURAL LOCALIZATION OF NEUROPEPTIDES IN THE RAT PRIMARY SENSORY NEURONES
- The Biologically Active Polypeptides in the Rat Central Nervous System
- Ultrastructural Localization of Substance P-, Somatostain-, Met-enkephalin- and β-endorphin-like Immunoreactivities in Rat Sensory Ganglia and in Cultured Dorsal Root Ganglion Cells
- IMMUNOHISTOCHEMICAL STUDIES ON THE DISTRIBUTION OF BIOLOGICALLY ACTIVE OLIGOPEPTIDES IN THE RAT CENTRAL NERVOUS SYSTEM
- Routine Ultramicro-Measurement of Human Growth Hormone in Plasma by Solid-Phase Radioimmunoassay
- Growth Hormone Releasing Factor Activity in the Stalk-Median Eminence and Plasma Growth Hormone Response to the Ether-Laparotomy Stress in the Rat
- Direct Inhibitions of Basal and Arginine-Induced Plasma Gastrin Secretion by Somatostatin in Anesthetized Dogs