Enzyme Immunoassay of Thyroxine in Serum and Dried Blood Samples on Filter Paper

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概要

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• A double antibody heterologous enzyme immunoassay was developed for measuring thyroxine (T<SUB>4</SUB>) in serum and in dried blood samples on filter paper for use in screening for neonatal hypothyroidism. In this method, ethanol extracts of 100 μ<I>l</I> of serum or dried blood spots of 9 mm in diameter were incubated with Tralkaline phosphatase conjugate prepared with glutaraldehyde, and antiserum to T<SUB>4</SUB>-bovine serum albumin conjugate prepared with carbodiimide. The enzyme activity in the precipitates formed with second antibody was then determined photometricallty at 500 nm. The measurable range of T<SUB>4</SUB> was 1-32μg/d<I>l</I>. A linear relation was obtained between the sample volume and the T<SUB>4</SUB> value, and a high correlation was found between values for T<SUB>4 </SUB>determined by this method and those for T<SUB>4</SUB> in serum determined by radioimmunoassay (coefficient of correlation: r=0.97 for serum and r= 0.88 for dried blood samples).

• 社団法人 日本内分泌学会の論文

著者

• Miyai Kiyoshi

  Central Laboratory For Clinical Investigation And Department Of Laboratory Medicine Osaka University

• ISHIBASHI KAICHIRO

  Eiken Immunochemical Laboratories

• KAWASHIMA MINORU

  Osaka Kessei Laboratories

• MIYAI KIYOSHI

  Central Laboratory for Clinical Investigation, Osaka University Medical School

関連論文

• FLUOROIMMUNOASSAY OF STEROID HORMONES
• Enzyme Immunoassay of Thyroxine in Serum and Dried Blood Samples on Filter Paper
• Radioimmunoelectrophoretic Analysis of Proteins Binding 3, 3, 5-Triiodothyronine in Human Serum
• Serum Thyrotropin Response to Thyrotropin Releasing Hormone and the Concentration of Thyroid Hormone in Patients with Hyperfunctioning Thyroid Nodule
• Relation Between Pituitary Responsiveness to Thyrotropin Releasing Hormone and Serum Thyroid Hormones in Hyperthyroidism

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