ガランタミンのケイ光定量

概要

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The authors found that 0.2N sulfuric acid solution of galanthamine gave fluorescence by the treatment with potassium permanganate, and attempted to use the reaction for the fluorometric determination. The relative fluorescence intensity was affected by the concentration of sulfuric acid in the solution, the amount of potassium permanganate, temperature and time of the oxidation. From the extensive studies of the above conditions, a recommended procedure was given, in which 10 ml of 0.2N sulfuric acid solution of galanthamine (0.22.0 γ/ml) were treated for 2 hours at 5 ± 1°C with 0.01N potassium permanganate, decolorized with 2.0 ml of 0.01N oxalic acid, kept overnight, and the relative fluorescence intensity was measured. A linear relationship was found between the concentration and the relative fluorencence intensity, although the slant was a little different on each set of calibration line. Determination of galanthamine by this method should be carried out together with a simultaneous treatment of a standard sample as the reference to assure an accuracy.
社団法人日本分析化学会の論文