生薬成分中のベルベリンの螢光定量

概要

論文の詳細を見る
An attempt has been made to establish a new method for determination of berberine, in which a strong yellow-green fluorescence given off by berberine on the filter paper under u. v. irradiation is utilized. The berberine was extracted from berberine-containing crude drugs, as Coptis and Phellodendron, with diluted acetic acid, and the absorption spectra and the fluorescence spectra were compared for the extract and a number of fractions thereof obtained by thepaper chromatographic separations. The fluorescence characteristics of berberine, the disturbing substances in crude drugs, and the conditions for the measurement of fluorescence intensity were widely investigated. In the recommended procedure, the extract of berberine fraction after a paper chromatographicseparation of the crude drug extract, or the solution of berberine powder diluted to a suitable concentration was spotted on the filter paper and the relative fluorescent intensity was estimated. As little as 0.11.0γ/spot of berberine was determined by this method.