ブホステロイドのシリカゲルによる薄層クロマトグラフィー

概要

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The activation of silica gel adsorbent and the efficacy of developing solvents in thin-layer chromatography of bufogenin were studied. The optimum activating conditions of silica gel depended more or less on its quality, but, generally, were 3060 min. standing at room temperature after spreading and the succeeding heat for 6090 min. The recommended developing solvents were; acetone-chloroform-cyclohexane (3 : 3 : 4), acetone-cyclohexane (3 : 7), <I>n</I>-hexane-ethyl acetate (1 : 9), and methanol-ethyl acetate-cyclohexane (2 : 6 : 7). Twenty-four bufogenins and sterins were detected and identified by the proper combination of the solvents or by the aid of two-dimensional development. The separation of 3-hydroxy isomer and 14-anhydro isomer and the determination of bufogenin were also investigated.
社団法人日本分析化学会の論文