A Simple, Large-Scale Overexpression Method of Deriving Carbon Monoxide Dehydrogenase II from Thermophilic Bacterium Carboxydothermus hydrogenoformans
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概要
- 論文の詳細を見る
We established an Na2S-free, large-scale overexpression system of deriving CODH II from thermophilic bacterium Carboxydothermus hydrogenoformans in Escherichia coli using a large-scale fermentor. Recombinant-CODH II showed a CO oxidation activity of 9,600 U/mg. In addition, recombinant-CODH II exhibited considerable CO2 reduction activity, of 16.9 U/mg.
- 社団法人 日本農芸化学会の論文
著者
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Yoshida Takashi
Division Of Breast Surgery Saitama Cancer Center
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Fukuyama Keiichi
Department Of Biological Sciences Graduate School Of Science Osaka University
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Sako Yoshihiko
Division Of Applied Bioscience Graduate School Of Agriculture Kyoto University
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Inoue Takahiro
Division Of Applied Biosciences Graduate School Of Agriculture Kyoto University
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Wada Kei
Department Of Biological Sciences Graduate School Of Science Osaka University
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DAIFUKU Takashi
Division of Applied Biosciences, Graduate School of Agriculture, Kyoto University
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