塩化鉄法によるコレステロールの比色定量に対するハイドロサルファイト効果について

概要

論文の詳細を見る
When a ferric chloride reagent was impure for colorimetric determination of cholesterol, the working line did not pass on its original point and thus had to be corrected suitably for the determination. This correction has been achieved by addition of hydrosulfite to the reagent without further purification. One milligram of hydrosulfite was added as a 15% water solution to 1 cc of the reagent. The reagent was found ready for use as a color reagent by keeping it for one day at 25° and also found stable at least for six days after the preparation. Whereas a reagent, if unstabilized, sometimes yields a turbid solution in the color reaction to render colorimetric measurement impossible. The time required to develop the maximum absorbancy of a color reaction mixture varies depending on preparations and therefore must be examined before drawing a working line.
公益社団法人日本水産学会の論文