鯨軟骨コンドロイチン硫酸のエタノールによる沈殿に関する研究-2-軟骨溶解液からの沈殿における塩の効果

概要

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The influences of salt and ethanol concentrations on the precipitation of chondroitin sulfate (CS), keratosulfate (KS) and protein in the Pronase digest of whale nasal cartilage were examined, and the following results were obtained. 1) CS was precipitated almost completely with 1.5 volumes of ethanol, whether the salt was added or not. 2) Some amounts of KS could not be precipitated even with 3 volumes of ethanol, remaining in ethanolic supernatant. 3) The higher the salt concentration or the lower the ethanol concentration, the less protein was precipitated. 4) There was little difference between sodium chloride and sodium acetate in their influence on the precipitation of CS, KS and protein. 5) The attempt to remove the greater part of contaminating protein from crude CS by the washing with 50-59% ethanol saturated with sodium acetate was successful.
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