サンマにおけるα-グリセロリン酸脱水素酵素の遺伝的多型-I. : 7変異型と遺伝的交配

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Seven different α-glycerophosphate dehydrogenase (α-GDH) isozyme patterns were electrophoretically observed in the white muscle cell-lysates of saury, Cololabis saira, from off the North Pacific coast of Japan and the East China Sea. The patterns indicated that they are controlled by four codominant alleles at an autosomal locus. The occurrence of hybrid isozymes in the heterozygotes suggests that the hybrid isozymes as well as other isozymes are of dimeric structure. Additional α-GDH isozymes which are controlled by at least two distinct loci were detected. It was found that a large scale screening of enzyme types for population analysis can be facilitated by the use of cell-lysates produced by thawing the frozen tissue. It is proposed that the dried cell-lysate-soaked filter paper and the tissue block taken in the agglutination test tray be used for population analyses of this kind because of the convenience in transporting and storing a large number of samples.
公益社団法人日本水産学会の論文