鉛による急性中毒死 (水質汚濁斃死魚の死因判定法について-10,11-)
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In this study, we attempted to detect lead in the body of rainbow trout killed by acute lead poisoning. Three ppm of a lead solution (lead nitrate) was found to be fatal to rainbow trout in 23-24 hours and the 48 h Tlm was found between 1 and 3 ppm Pb. It was found that the nature of the water used for preparing the lead solution influences considerably the toxicity of lead. The use of hard water (2.0 meq/l alkalinity) resulted in a great reduction of the toxicity (Table 1). Lead content of tissues was determined by the Diphenylthiocarbazone method. Lead levels of the control fish were less than 0.34 γ/g Pb and 0.25 γ/g Pb on an average (Table 2). Lead content of fish killed by lead solutions was higher than that of the control fish (Table 3). When the body of fish was washed with running tap water for 24 hours after death, lead content decreased to 60.2-95.5% of the initial values (Table 5). Seven parts of the fish body were examined for distribution of lead in two cases. In the first case, fish were killed by a lead solution and in the 2nd, fish were soaked in a lead solution after death by suffocation. In both cases the gills showed the highest value of lead content among the seven parts and the muscles showed the lowest. Lead content of gill was higher in the first case than in the 2nd case (Table 6). It was of interest to not that lead was detected scantily in liver and alimentary canal in the 2nd case. It has to be pointed out that in the diagnosis of lead poisoning it is important to know the distribution of lead in the carcass.
- 社団法人 日本水産学会の論文
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