キリンサイ(Eucheuma muricatum (GMEL.) WEBER VAN BOSSE) 粘質に関する研究-VI : 構成糖組成について

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As shown in Fig. 1, the sugar solution obtained by hydrolysis of one gram mucilage (Basalt) with dilute sulfuric acid was neutralized and decolorized, and separated into acidic and neutral sugar using ion-exchangers as previously reported. The neutral sugar fraction was subjected to liquid chromatography by passing butanol, half-saturated with water, through a cellulose column (2×49.5cm) packed with pure cellulose prepared from filter paper pulp, and it was thereby separated into three components: anhydrogalactose, xylose and galactose. The methods employed for the estimation of sugars, acidic and neutral, and the results obtained (tabulated in Table 1) were as follows: (1) Glucurone was determind by the WILLIAMS color reaction, since the optical density at 600mμ corresponds to the concentration of glucurone (Fig. 2), and was found to be present by 3.27% on the total sugar basis. (2) Galactosesulfate was calculated by subtracting the above glucurone content from whole acidic sugar content as shown in Table 1, and was found to be contained five times as much as glucurone was. (3) Each component of neutral sugars was estimated by the hypoiodite method. It was found that total neutral sugar amounts to about 80% of total sugar, and the proportions of xylose to anhydrogalactose and galactose become nearly 1:3.5:12.5. (4) As the value obtained by the hypoiodite method coincides satisfactorily with that of solid matter and the recovery of total sugar is 96% (in the case of hypoiodite method) or 99% (in the case of solid matter), it may be declared that each sugar is separated from Eucheuma mucilage in a pure state and quantitatively by this method.
公益社団法人日本水産学会の論文

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中村武彦
宮崎大学農学部

キリンサイ(Eucheuma muricatum (GMEL.) WEBER VAN BOSSE) 粘質に関する研究-VI : 構成糖組成について

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