Studies on Mucilage from the Seaweed, Eucheuma spinosum (L.) J. Ag.-III:Studies on Component Sugars of Cold Extract

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Cold extract was obtained from 1kg. of the alga by the method adopted in the previous paper. Nitrogen-free barium salt was prepared from filtrate of the above extract, with the ratio of sugar residue to combined sulfate as 1:1. With a view to clarify sugar components of this salt, hydrolysation with 3% H2SO4 in a water bath for 2.5 hours was followed by complete neutralization with Ba(OH)2 solution. The fluid was thickened to a consistency of syrup in vacuo at temperatures below 40°C. The syrup was ascendingly chromatographed on No. 2 Toyo Filter Paper. Three spots were made visible on the paper with P-anisidinehydrochloride as tabulated in table 1. Chromatographical separation of component sugars was done as follows: First, take a sheet of the filter paper 30cm. in width. The syrup was drawn on it as a transverse strip 3mm. in width at a height of 6cm. from the lower edge. Then it was chromatographed as mentioned above. After a lapse of 24 hours, three locations (upper, middle and lower) were cut off according to Rf values. Hot water extractions were identified by color reaction, chromatography or some other methods. (Table 2 and 3). The results obtained may be summarized as follows: (1) The upper represents an unknown sugar. This is not fructose although it gives SELIWANOFF ketose reaction. An attempt to prove it as anhydro-galactosephenylosazone has been futile. (2) The middle is likely to represent glucuron since it shows DISCHE's reactions (Figs. 1 and 2). (3) The lower corresponds to galactose since it gives α-methylphenylhydrazone (m. p. 190°). It may be mentioned that proportion of glucuron to anhydro-sugar (assumed), and galactose nearly runs as 1:3:12.
社団法人日本水産学会の論文

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中村武彦
宮崎大学農学部

Studies on Mucilage from the Seaweed, Eucheuma spinosum (L.) J. Ag.-III:Studies on Component Sugars of Cold Extract

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