Studies on Mucilage from the Seaweed, Eucheuma muricatum (GMEL.) WEBER VAN BOSSE-V:Identification of Glucuronic Acid
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Glucuronic acid or its lactone (glucurone) is nowadays recognized as one of the sugar components of mucilages from seaweed.(1), (2) The detection of glucurone by paper chromatography was very difficult as reported in the previous paper.(3) In the present paper, several color reactions for glucuronic acid were applied to the acidic fraction separated with ion-exchangers from the hydrolysates of Basalt of the mucilage. The following results were obtained: (1) The most reliable and sensitive test was found to be the WILLIAMS reaction.(5) The color formed showed a characteristic absorption maximum at 600mμ. (Fig. 2). (2) DISCHE's thioglycolic acid (6) and cysteine reactions (7) were also useful for detection, but not for determination, because the color intensity was markedly affected by the conditions in treating with conc. H2SO4 (Fig. 3, 4). (3) The original naphthoresorcinol reaction (TOLLENS)(4) gave a characteristic absorption curve which has a maximum peak at 559mμ, but it was less sensitive than the WILLIAMS reaction. This reaction seems to be inapplicable for the determination, because the extraction of the color substance was not quauntitative. (Fig. 1). (4) The JARRIGE's reaction(5) seemed to be inadequate for spectrophotometric investigation due to the formation of turbid coloration. (5) The carbazol reaction(8) was found to be not specific for glucuronic acid and the similar absorption curves were obtained on xylose, glucose and galactose (Fig. 5). Thus, glucuronic acid or glucurone in the acidic fraction of Eucheuma muricatum was clearly identified by the WILLIAMS, DISCBE's and original naphthoresorcinol reactions, and its content was 3.27% on the dry basis of the hydrolysates, when determined by the WILLIAMS reaction.
- 社団法人 日本水産学会の論文
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