Cotton Acid Succinateによるヒスタミンの定量について

概要

論文の詳細を見る
MCINTIRE's method for the purification of histamine was applied to the quantitative determination of histamine produced in decomposed meat extract of fish. It was found that this method is very suitable for rapid determination of histamine in a large, number of samples. The essential features of this method are as follows: A decomposed meat extract of fish which contains histamine is extracted with n-butanol under conditions such that 83% of the histamine is removed from the apueous phase in one extraction. The histamine is recovered from the butanol by means of a new cation exchange medium, cotton acid succinate. The histamine is eluted from cotton acid succinate with a small volume of diluted hydrochloric acid and the elute is neutralized with sodium carbonate and diluted with water to give a suitable concentration for colorimetrical determination with diazo-reaction.
社団法人日本水産学会の論文