アミノ酸自動分析計による有効性リジンの定量

概要

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A chemical method for the determination of available lysine in protein based on the reactivity of dinitrofluorobenzen (FDNB) with free ε-amino group of lysine has been reported by CARPENTER. RAO et al. and NIELSEN et al. used the CARPENTER hydrolysis technique followed by ion-exchange chromatography to measure the available lysine directly. In this paper, the amount of available lysine was measured from the determination of total lysine and residual one of FDNB treatment by using an amino acid analyzer. The technique is seen to be convenient, and it gave more satisfactory results as compared with that obtained by the method of CARPENTER. The direct determination of ε-DNP-lysine by using amino acid analyzer was also described.
社団法人日本水産学会の論文