鯨膵臓の酵素利用について-1-

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This study was undertaken to re-examine the widely accepted view that the pancreas of whale has far lower proteolytic enzyme activity compared with those of domestic animals. Pancreas of two kinds of whale, fin whale and sperm whale, were minced, freeze-dried, defatted with ether, and pulverized. Samples thus obtained were then analyzed for proteoly-tic activity under various conditions according to the Japan. Pharmacopoeia7). It was found that the fin whale sample needs fairly longer time for activation of proteo-lytic enzyme than land mammalian ones, and is not essentially different in the final activity from the latter, though the sperm whale sample shows only poor activity even after far longer time of standing (Fig. 1). Effect of the concentration of the sample upon the activation is remarkable (Fig. 2). Effect of several inorganic ions upon the activation was also examined: KCI, NaCl, CaCO and CaSO, accelerate to some extent the activation, while the others, mainly heavy metal salts, markedly inhibit it (Figs. 3 and 4). Then some experiments were carried out in effort to shorten the activation time of pro-teolytic enzyme. Neither the autolysis of, nor the additions of enterokinase and the whale small intestines to, the minced pancreas, seems not very effective in this respect (Figs. 5-7). Addition of the pig small intestines is, however, so effective that the maximum activity can be reached within 1 hr. after standing (Fig. 8).
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鯨膵臓の酵素利用について-1-

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