水産動物のキチン分解酵素に関する研究-6-ニジマスのキチン分解酵素について

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In the previous papers1-5) it was reported by the present authors that the chitinolytic activity is present in some organs of a few kinds of aquatic animals. The present study was carried out in order to ascertain whether similar chitinolytic activity is present in some organs of rainbow trout, Salmo irideus. Some properties of the chitinolytic enzyme were investigated by measuring total N-acetylamino sugar liberated in the reaction mixture using precipitated chitin (P. Ch) as the substrate. Results obtained were as follows; 1) The chitinolytic enzyme is present in the stomach of this fish in high activity and present in the intestine, the gall bladder and the liver in moderate to slight activity, but not in the pyloric caeca and spleen at all (Table 1). 2) The optimum pH value of this enzyme action is about 4.5 (Fig 2), and the optimum temperature is about 30°C (Fig. 4). 3) Between pH 3.0 and 9.0, the activity of this enzyme is gradually decreased by in-creasing the pH value (Fig. 3). 4) This enzyme is stable in 20min. standing at 30-60°C, while not above 60°C, instantly losing almost all the activity at 80°C (Fig. 5).
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