暖冬期における寒天の変敗防止に関する研究-XI : “ダンカン”現象に関与する細菌群の性状について

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The distribution and the classification of bacteria concerning the spoilage of “tokoroten”and agar during the manufacturing process in mild winter were studied. The results obtained are shown in Tables 1-10, and summarized as follows: 1. Implements, water, material algae used for the manufacture, spoilt “tokoroten” and degraded agar were found to be contaminated with a large number of bacteria including agar-decomposing ones. 2. As agar-decomposing bacteria 46 strains were isolated and they were classified into 2 genera-4 species according to BERGEYs Manual 7) and H. J. HUMM 8), namely, Pseudomonas lacunogenes, Pseudomonas segnis, Pseudomonas perfectomarinus and Bacterium rhodomelae. Of the species mentioned above, Pseudomonas lacunogenes appeared to play an important role in the spoilage of “tokoroten” and agar. 3. As chromobacteria 45 strains belonging to 5 genera-14 species were isolated, namely, Flavobacterium lactis, Flavobacterium esteroaromaticum, Flavobacterium solare, Flavobacterium harrisonii, Pseudomonas solaniolens, Pseudomonas salopium, Pseudomonas radiciperda, Pseudomonas melophthara, Corynebacterium agropyri, Corynebacterium michiganense, Bacterium imperiale, Micrococcus cinnabareus, Micrococcus luteus, Micrococcus varians. Among these 14 species Flavobacterium lactis which was most predominat and actively produced pigment, may be causative of the coloration of degraded agar.
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