水産動物のコラーゲンに関する研究-I : 酵素処理法によるコラーゲンの精製条件の検討

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The study was planned for the purification of collagen in dolphin hide by enzymatic treatment without destructing the fibre structure, the following result being obtained. The Hiirotake enzyme product containing an acid-active enzyme from Trametes sanguinea (L. ExFr) Lloyd, seems likely to be capable of attacking the collagen from the fact of appearance of free hydroxyproline (Table 2). On the contrary the Bioprase, an alkali-active enzyme obtained from Bac. subtilis var. Biotecus gave free hydroxyproline to an extent slightly observed after quite a long treatment (Tables 3 and 4). Considering from the amounts of free amino acids and saccharides appearing in solution (Anthron and Orcin method), a time of liming may be said to be effective enough of two weeks. From the fact that the increase of free amino acids and saccharides does not readily reach to equilibrium the enzymatic treatment can hardly be regarded as a satisfactory method of purification of collagen.
公益社団法人日本水産学会の論文