Decomposition of Glucosamin by Pseudomonas fuorescens

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On the decomposition of glucosamin (which is a decomposed product from chitin as anim-portant component in the shell of Crustacea) by bacteria, only a few papers(1, 2, 3) have been published. In the present experiment the synthetical medium, which was previously reported by the author(13), was used. To 500cc of this medium 4gr. of glucosaminhydrochloride were added and sterilized. Pseudomonas fluorescens was cultivated in this medium for 3 weeks at 28°C. Then the decomposed products were isolated from the culture, which was altered brownish in colour day by day. Methylglyoxal was determined by the NEUBERG and KOBEL's(4, 5), BARRANSCHEEN and BRAUN's(6), and SIMON and NEUBERG's(7) methods. SIMON and NEUBERG's(7), NEUBERG and KOBEL's(8), and CASE's(9) methods were employed for determing pyruvic acid. Volatile acids were obtained by means of the steam distillation. Lactic acid and malic acid were obtained by means of the ether-extraction from the medium, which was distilled beforehand in order to isolate pyruvic acid from it. From the results of experiment, it is clear that methylglyoxal, pyruvic acid, lactic acid and malic acid were produced from glucosamin by the action of Psudomonas fluorescens. Moreover. probably formic acid and acetic acid were formed as the decomposition products.
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