Antagonism of Bacteria in Relation to Water-Content of the Culture Media

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Various authors have studied antagonism of the bacteria, since BLAGOVESTSCHENSKY discovered that growth of Bacillus anthracis is suspended when this organism is cultured together with Pseudomonas pyocianea. And yet none has cleared up the cause of this antagonism. Several kinds of atmospheric as well as aquatic bacteria were cultured either separately or mixedly in the media consisting of varying percentages of common broth and glycerol. Glycerol was used to modify the water-content of the media and was not attacked by any of the micro-organisms tested. After a definite incubation time, a definite volumes of the cultured organisms were put into the agar media. And agar plate cultures were made by the shaking method. Results obtained are shown in Figs. 1 and 2, in which the numbers denote the percentages of water content of the media. From the figures it is clear that the characteristic rate of reproduction of each species of the experimental bacteria depends on the water-content of the medium and that antagonism of the bacteria is either absent or present to varying extent according to the kinds of the organisms tested.
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