Essential Role of Extracellular Ca2+ for the Maintenance of the Ca2+ Activated K+ Channel Activity in Guinea Pig Vas Deferens.
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概要
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The characteristics of Ca2+ activated K+ channels of enzymatically dissociated single smooth muscle cells of vas deferens of the adult guinea pig were studied by the whole cell clamp technique. A stepwise depolarization to -30mV induced a fast transient current followed by the spontaneous transient outward currents (STOCs). STOCs were eliminated by application of K+ channel blocker TEA. STOCs appeared only when Ca2+ was present in extracellular medium, and disappeared immediately once Ca2+ was removed from the medium. Therefore, STOCs in the present preparation can be ascribed to the activity of Ca2+ activated K+ channels. Furthermore, even in the absence of Ca2+, STOCs were evoked transiently by application of caffeine, indicating the involvement of the Ca2+ induced Ca2+ release (CICR) mechanism. Thus, it is likely that Ca2+ activated K+ channels are operated via CICR mechanism and extracellular Ca2+ is essential for keeping their constant activity.
著者
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Kato Kenichi
Department Of Cardiovascular Medicine Okayama University Graduate School Of Medicine Dentistry And P
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Tsutsui Izuo
Department Of Cell Physiology National Institute For Physiological Sciences
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YAMAGISHI Shunichi
Department of Cell Physiology, National Institute for Physiological Sciences
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