N-Ethylmaleimide Blocks the H^+ Pump in the Plasma Membrane of Chara corallina Internodal Cells
スポンサーリンク
概要
- 論文の詳細を見る
The sulthydryl (SH) modifying reagent N-ethylmaleimide (NEM) was applied to the inter-nodal cells of Chara corallina to study the role of SH residues in the activity of the plasma membrane H^+ pump. NEM (1μM) caused a marked depolarizing shift of the resting potential by 64±10 mV (n=7) together with depression of the conductance peak at around -200 mV, indicating a marked depression of the H^+ pump activity. This effect of NEM was partly reversible, the membrane repolarized and the conductance peak was restored after extracellular washing. The H^+ pump inhibitor, dicyclohexylcarbodiimide (DCCD), caused no additive membrane depolarization and/or depression of the H^+ pump conductance, in the presence of NEM. This suggests that NEM blocks the H^+ pump and that SH residues play a pivotal role in maintaining the H^+ pump activity in Chara corallina.
- 日本植物生理学会の論文
著者
-
Tsutsui Izuo
Department Of Cell Physiology National Institute For Physiological Sciences
-
OHKAWA Takaaki
Department of Biology, College of General Education, Osaka University
-
Ohkawa Takaaki
Department Of Biology College Of General Education Osaka University
関連論文
- A Ca^-and Voltage-Dependent Cl^--Sensitive Anion Channel in the Chara Plasmalemma: A Patch-Clamp Study
- Effects of Divalent Cations on the Excitability and on the Cytoplasmic Streaming of Chara corallina
- Inhibition of Cl^- Channel Activation in Chara corallina Membrane by Lanthanum Ion
- Early Events in Plasma Membrane of Chara corallina after Electrical Stimulation. I.Studies under Current Clamp Conditions : MEMBRANES AND BIOENERGETICS
- K^+ Channel in the Chara Plasmalemlna : Estimations of K^+ Channel Density and Single K^+ Channel Conductance
- Effects of Calmodulin of Ca^-Dependent Cl^--Sensitive Anion Channels in the Chara Plasmalemma : a Patch-Clamp Study
- N-Ethylmaleimide Blocks the H^+ Pump in the Plasma Membrane of Chara corallina Internodal Cells
- Light-Regulated Translocation of Cytoplasm in Green Alga Dichotomosiphon
- Essential Role of Extracellular Ca2+ for the Maintenance of the Ca2+ Activated K+ Channel Activity in Guinea Pig Vas Deferens.