ATTEMPTS TO APPROACH TO THE CLARIFICATION OF CHEMICAL COMPOSITION OF MYCOBACTERIUM LEPRAEMURIUM III. CHEMICAL COMPOSITION OF THE BACILLI SEPARATED FROM THE SUBCUTANEOUS TISSUE OF RAT INFECTED WITH MYCOBACTERIUM LEPRAEMURIUM BY PRETREATMENT WITH TRYPSIN
スポンサーリンク
概要
- 論文の詳細を見る
In the first report (1) (2) (3), a separation method of Myc, lepraemurium from the infected subcutaneous tissue of rat by means of chloroform fractionation, and in the second report (4) (5), some chemical properies of the bacilli, i. e, their nitrogen, phosphorus, amino acids, and carbohydrate contents, and alcoholether extractible fraction were described. However, the data of the chemical properties of the bacilli in these papers may not be natural in their state, because the chloroform was used as a fractionation solvent for the separation of the bacilli from the infected tissue, and many fractions in the bacilli seemed to be extracted into the chloroform layer, and moreover, the infectivity of the bacilli to rat was lost by this fractionation procedure. After the publication of these reports, the author received a communication from Dr. H. W. Wade (6), Culion, Philippines, which stated that chemical analyses of bacilli separated from the tissue by use of a solvent like chloroform could not represent the bacilli in their natural state for the reason of electron microscopic observations by Dr. K. R. Chatterjee and others in which the bacilli separated from the tissue by chloroform were so much altered that their pictures were valueless. Therefore, the efforts toward the isolation method of Myc. lepraemurium from infected subcutaneous tissue in the natural state were made.The purpose of this present paper here is to report a new isolation method of murine leprosy bacilli from infected tissue and comparison between the chemical analyses of the bacilli obtained by this method and those of the bacilli obtained by the chloroform fractionation. The former is a method in which pretreatment of infected tissue with trypsin is employed before Hanks procedure (7) by which the intact cells of murine leprosy bacilli can be effectively obtained from the infected tissue, for a solution of difficulty to separate the leprosy bacilli from the infected subcutaneous tissue.After expressing this method at the 29th Japanese Leprosy Congress, 1956 (8), a communication from Dr. J. H. Hanks (9), Harvard Univ. U. S. A. in which pancreatin and bile were used for facility of separation of murine leprosy bacilli from the tissue and a communication from Dr. C. M. Carpenter (10), Univ. California, U.S.A. in which trypsin could be effectively employed to separate tissue components from suspensions of Myc. leprae prepared from lepromata for obtaining a tissue-free lepromin, were received.
- 久留米大学医学部 The Kurume Medical Journal 編集部の論文
著者
-
Nakamura Masahiro
Department Of Agronomy Facluty Of Agriculture Tohoku University:(present Address) Miyagi Agricultura
-
NAKAMURA MASAHIRO
Department of Microbiology, Kurunme University, School of Medicine
関連論文
- Transition Enthalpy for a Poly(N-isopropylacrylamide) Based Polymer Gel Estimated from the Clapeyron Equation
- KADISON'S SCHWARZ INEQUALITY AND FURUTA'S THEOREM
- GRAPHS AND KOLMOGOROV'S COMPLEXITY
- Localization of Aldosterone-Producing Adrenocortical Adenomas : Significance of Adrenal Venous Sampling
- A New Physiologically Based Pharmacokinetic Analysis for In Vivo Organ Disposition of Nitroxyl Spin Probes in Mice as Measured by Multisite Detection L-band ESR (MSD-LESR)
- Identification of Bombyx mori Midgut Receptor for Bacillus thuringiensis Insecticidal CryIA (a) Toxin
- ANDO'S THEOREM FOR HADAMARD PRODUCTS AND OPERATOR MEANS
- OPERATOR VALUED DETERMINANT AND HADAMARD PRODUCT
- Postharvest Berry Drop of Seedless Berries Produced by GA Treatment in Grape Cultivar 'Kyoho' IV. Rachis Hardness and Phenol Production of Rachis in Vitro Following GA Application as Affected by the Addition of β-Nitrostyrene Derivatives
- Postharvest Berry Drop of Seedless Berries Produced by GA Treatment in Grape Cultivar 'Kyoho' III. Effect of GA on Xylem Differentiation and Lignification of Rachis
- Postharvest Berry Drop of Seedless Berries Produced by GA Treatment in Grape Cultivar 'Kyoho' I. Relationship between Postharvest Berry Drop and Rachis Hardness
- Postharvest Berry Drop of Seedless Berries Produced by GA Treatment in Grape Cultivar 'Kyoho' II. Relationship between Rachis Hardness and Differentiation of Rachis Xylem
- KADISON'S SCHWARZ INEQUALITY AND NONCOMMUTATIVE KANTOROVICH INEQUALITY
- JENSEN INEQUALITY IS A COMPLEMENT TO KANTOROVICH INEQUALITY
- APPROXIMATION TO THE SQUARE ROOT OF A POSITIVE OPERATOR
- RICCATI EQUATION AND THE FIEDLER-PTAK SPECTRAL GEOMETRIC MEAN
- SPECTRAL ORDER OF OPERATORS AND FURUTA INEQUALITY
- Large p_T Jet Production and Hard Rearrangement of Quarks
- Kadison’s Schwarz and Kantorovich inequalities on correlation operators
- Oxidative Coupling Polymerization of 2, 6-Dimethylphenol with a Copper-Amine Catalyst Immobilized within the Interior of SBA-15
- OPERATOR MEAN AND KANTOROVICH INEQUALITY
- CONDITIONAL EXPECTATION AND HADAMARD PRODUCT OF OPERATORS
- AN APPLICATION OF OPERATOR MEANS ARISING FROM A STUDY ON WAZAN
- An extension of Kantorovich inequality
- WIGNER'S WEAKLY POSITIVE OPERATORS
- COOPER'S APPROACH TO CHAOTIC OPERATOR MEANS
- Elementary Proofs of Gleason-Kahane-Zelazko's Theorem for B-algebras
- A CHARACTERIZATION OF THE HARMONIC OPERATOR MEAN AS AN EXTENSION OF ANDO'S THEOREM
- Ergodic Theorems for Algorithmically Random Sequences
- REMARK ON CHAOTIC FURUTA INEQUALITY
- EFFECTS OF RECEPTOR-DESTROYING-ENZYME ON THE VIRUS HEMAGGLUTINATION USING ERYTHROCYTES TREATED WITH AND WITHOUT FORMALIN
- SOME PROPERTIES OF MOUSE ENCEPHALOMYELITIS VIRUS; ESPECIALLY HEAT, ETHER, AND ACETONE STABILITIES OF THE VIRUSES
- THE MODIFYING EFFECTS OF BACTERIAL SUBSTANCES IN THE COURSE OF INFECTION WITH NEUROTROPIC VIRUS:II. REPORT. THE INHIBITORY EFFECT OF BACTERIAL SUBSTANCES RELATED E. COLI ON THE NEUROTROPIC VIRUS INFECTION
- Ultraviolet irradiation and photoreactivation in mycobacteria.
- GROWTH FEATURES OF MYCOBACTERIUM LEPRAEMURIUM CULTIVATED IN NC-5 MEDIUM
- IMPROVEMENT TO THE NC-5 MEDIUM FOR CULTURING MYCOBACTERIUM LEPRAEMURI UM (ND-5 MEDIUM)
- Stimulating effect of soluble starch on the growth of Mycobacterium lepraemurium in cell-free liquid medium; preliminary report.:PRELIMINARY REPORT
- ATTEMPTS TO APPROACH TO THE CLARIFICATION OF CHEMICAL COMPOSITION OF MYCOBACTERIUM LEPRAEMURIUM:II. Some properties of the bacilli from the subcutaneous tissue of rat infected with Mycobacterium lepraemurium
- Effect of Molecular Weight of Polyethylene Glycols on Inactivation of Mycoplasma pulmonis and Acholeplasma laidlawii A
- Semisolid culture medium for oxidative fermentation test of Pseudomonas aeruginosa.
- MYCOPLASMACIN : MYCOPLASMA GROWTH INHIBITORY SUBSTANCE PRODUCED BY MYCOPLASMAS
- Survival of mycoplasma, acholeplasma and spiroplasma at different temperatures.
- ATTEMPTS TO APPROACH TO THE CLARIFICATION OF CHEMICAL COMPOSITION OF MYCOBACTERIUM LEPRAEMURIUM III. CHEMICAL COMPOSITION OF THE BACILLI SEPARATED FROM THE SUBCUTANEOUS TISSUE OF RAT INFECTED WITH MYCOBACTERIUM LEPRAEMURIUM BY PRETREATMENT WITH TRYPSIN
- PRESERVATION OF MYC. LEPRAEMURIUM BY MEANS OF LYOPHILIZATION II REPORT
- STAINING CONDITIONS INFLUENCING MORPHOLOGICAL INDEX OF ACID-FAST BACILLI
- THE MODIFYING EFFECTS OF BACTERIAL SUBSTANCES IN THE COURSE OF INFECTION WITH NEUROTROPIC VIRUS:I. REPORT, AN EXPLORATORY EXPERIMENT (I)
- Selection and propagation of antibiotic-resistant mutants of Acholeplasma by the addition of fresh culture medium containing antibiotics and protease to the medium cultured with Acholeplasma.
- EFFECT OF THALLIUM ACETATE ON THE GROWTH OF BACTERIA