有菌と無菌環境下での飼育ラットの反転腸管における15N-アンモニアの吸収

概要

論文の詳細を見る
Camparative experiments of ammonia absorption were undertaken by the Wilson and Wiseman methods with everted sacs of the middle part of intestine and the cecum from seven-week-old germfree (GF) and conventianal (CVZ) rats fed on a commercial diet CL-2 (Clea Japan, Inc.) sterilized by auto-claving. The ammonia concentration ratio of serosal fluid (SF) to mucosal fluid (MF), i. e. SF/MF ratio in case of the middle gart of small intestine showed that the ammania absorption rate of GF rats was significantly higher than that of CVZ rats when the initial concentration of ammania in MF was 1 mM. This finding was verified by other experiments of <SUP>15</SUP>N-ammonia absorption. Results of SF/MF ratios obtained by experiments of the cecum were uncertain.