Generation of lymphokine activated killer cells from CD 4-negative peripheral blood lymphocytes using the flask immobilized with anti-CD3 antibodies.

概要

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In the generation of lymphokine activated killer (LAK) cells from peripheral blood lymphocytes (PBL), large numbers of LAK cells are obtained by culturing in the anti-CD 3 immobilized flasks. However, the killer activity of the LAK cells which were cultured in these flasks (PBL·anti CD 3-LAK) is low. In this study, CD 4-positive and -negative PBL were cultured. CD 4+-LAK cells which were generated from CD 4+ PBL exhibited very low killer activity, although the cell proliferation was explosive. Whereas, CD 4--LAK cells generated from CD 4- PBL showed high killer activity. CD 4--LAK cells demonstrated high adhesiveness against the cancer cell line T 98 G, CD 4+-LAK cells having no any adhesive tendency. These results may show that the low killer activity of PBL·anti CD 3-LAK is due to the explosive proliferation of CD 4+-LAK cells. CD 4--LAK cells include CD 4- CD 8--and CD 4- CD 8+-LAK cells. CD4-CD8--LAK cells showed very high killer activity, and CD 4- CD 8+-LAK cells relatively high activity. The culturing of CD 4- PBL in the anti-CD 3 immobilized flasks is useful to obtain a lot of LAK cells by accompanied high killer activity.
日本臨床免疫学会の論文