Measurement of cytoplasmic viscosity with fluorescence polarization technique in lymphocytes, unstimulated & stimulated with phytohemagglutinin.

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概要

• 論文の詳細を見る

• The fluorescence polarization technique to measure cytoplasmic microviscosity of human peripheral lymphocytes was established. The previous method, which was proposed by Cercek, has been corrected because a serious mistake was revealed in the process of cell filtration. The mean value of fluorescence polarization in lymphocytes from 20 healthy donors was 0.190±0.005. The mean value of fluorescence polarization as % of control after stimulation of lymphocytes with phytohemagglutinin (PHA) was 83.6±3.8. The optimal conditions in the measurement were discussed.

• 日本臨床免疫学会の論文

著者

• 橋本 康男

  横浜船員保険病院

• 篠崎 直美

  横浜船員保険病院内科

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• Measurement of cytoplasmic viscosity with fluorescence polarization technique in lymphocytes, unstimulated & stimulated with phytohemagglutinin.

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