Studies on the heterogenity of cytotoxic lymphocytes generated by mixed culture with autologous Epstein-Barr virus-transformed cell line:With special reference to Interleukin-2-Activated Killer

スポンサーリンク

概要

• 論文の詳細を見る

• Cytotoxicity against both Natural Killer (NK)-sensitive and NK-resistant targets can be induced by mixed lymphocyte culture reaction (MLR) and is referred to NK-like activity. Short-term cultures of mononuclear cells with recombinant interleukin-2 (IL-2) result in the appearance of considerable cytotoxicity against various targets (IL-2-activated killer, Lymphokine-activated killer (LAK)). This work intends to investigate the spectrum and specificity of these two cytotoxicities. When stimulated in vitro for 6 days with autologous Epstein-Barr virus-transformed lymphoblastoid cell line (LCL), the lymphocytes from Epstein-Barr virus seropositive human donors became cytotoxic for K 562, Molt-3 (NK-sensitive targets), Raji (NK-resistant target) and allogeneic LCL as well as autologous LCL. In contrast, IL-2-activated killer cells were cytotoxic also for K 562, Molt-3, Raji and allogeneic LCL, but not very much for autologous LCL. The cold-target inhibition test was used to analyze the specificity of these two cytotoxicities and revealed the similarity of them for all target cells tested. The cytotoxicity against NK-sensitive targets (K 562, Molt-3) was inhibited only by K 562 and Molt-3, which indicated that K 562 and Molt-3 have both NK and LAK target structures, but other cells only LAK target structure or alternatively that the wide spectrum effector cells have multiple receptors. The cytotoxicity against Raji was strongly inhibited by not only Raji but also K 562 and Molt-3, but not by LCL. Therefore Raji seemed to have LAK target structure of wider spectrum than that of LCL or to be killed by the effectors which have multiple receptors. The cytotoxicities against allogeneic LCL or autologous LCL were also inhibited by K 562, Molt-3 and Raji as well as target itself. The activated "NK cells" and the killer cells; LAK or LAK-like cells, with broad target spectrum including NK-resistant cells, are probably involved in these cytotoxicities. The cytotoxicity against autologous LCL was induced by MLR with autologous LCL, but not very much by IL-2. This suggested the presence of LAK-like cells not induced by IL-2. The biological role of these cytotoxicities is not yet well known. However, the cytotoxicity against autologous LCL by LAK or LAK-like killer cells with broad spectrum may be functioning in immune surveillance for Epstein-Barr virus-transformed cells.

• 日本臨床免疫学会の論文

著者

• 高木 秀二

  東京医科歯科大学医学部小児科

関連論文

• 骨髄移植後に Epstein-Barr virus に関連した lymphoproliferative disorder を発症した Wiskott-Aldrich 症候群の1例
• 健常な皮膚と頭髪からの真菌の検出
• 374 EBVに対するシェーグレン症候群患者の免疫能の検討
• タイトル無し
• Studies on the heterogenity of cytotoxic lymphocytes generated by mixed culture with autologous Epstein-Barr virus-transformed cell line:With special reference to Interleukin-2-Activated Killer

スポンサーリンク