Thymocyte Transfer to Enucleated Oocytes in the Mouse

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概要

• 論文の詳細を見る

• Thymocytes from fetal, newborn, and adult mice were transferred into enucleated oocytes by standard micromanipulation. The developmental ability of oocytes receiving a thymocyte was examined <I>in vitro</I>. Most of the reconstituted eggs were successfully activated and formed a pronucleus-like structure. The percentage of reconstituted eggs developing to the 2-cell stage was high, 75-84%, but development of embryos to more advanced stages was limited. Only 8 and 1% of the reconstituted eggs receiving a thymocyte from fetal and newborn mice developed to the blastocyst stage, respectively. None developed to the 8-cell stage when a thymocyte from adult mice was transferred. Analysis of newly synthesized proteins of the reconstituted embryos showed that 68-70 kDa heat shock proteins, initial markers of embryonic genome activation, were synthesized at the late 2-cell stage.

• 日本繁殖生物学会の論文

著者

• Kono Tomohiro

  Nodai Research Institute Tokyo University Of Agriculture

• Nakahara Tatsuo

  Nodai Research Institute Tokyo University Of Agriculture

• OGAWA Miyoko

  NODAI Research Institute, Tokyo University of Agriculture

関連論文

• Effects of Trophectoderm Dissection on Development of Inner Cell Mass of Fresh and Frozen-Thawed Bovine Blastocysts Derived from In-Vitro Fertilization
• Activation of Mouse Oocytes by Transferring a Nucleus from Fertilized Embryos
• DNA Synthesis in Mouse 1-Cell Embryos Containing Transferred Nuclei
• Production of Live Young by Serial Nuclear Transfer with Mitotic Stages of Donor Nuclei in Mice
• Thymocyte Transfer to Enucleated Oocytes in the Mouse

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