Activation of Mouse Oocytes by Transferring a Nucleus from Fertilized Embryos

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概要

• 論文の詳細を見る

• Transferring the nucleus from a fertilized mouse 2-cell embryo causes oocyte activation. Here, the sequential changes in a transferred nucleus in reconstructed oocytes were investigated and the histone H1 kinase activity of these oocytes was studied. Diploid nuclei from fertilized or parthenogenetic 2-cell embryos placed in oocytes underwent nuclear envelope breakdown (NEBD) and premature chromosome condensation (PCC) and the histone H1 kinase activity was high within 1 h after fusion. Two polar bodies were extruded when the oocytes received fertilized 2-cell nuclei, and two pronuclei were formed 5 to 6 h after fusion. Histone H1 kinase activity then significantly decreased and reached the basal level (35.6% of the initial level). However, oocytes receiving a nucleus from parthenogenetic 2-cell embryos arrested at metaphase, although histone H1 kinase activity was diminished only slightly (71.6% of the initial level). These results showed that nuclei from early fertilized embryos induced oocyte activation and that various events in oocytes activated by these nuclei were similar to fertilization in some respects.

• 日本繁殖生物学会の論文
• 1996-08-01

著者

• Kono Tomohiro

  Nodai Research Institute Tokyo University Of Agriculture

• Obata Yayoi

  東京農業大学 バイオサイエンス学科

• Obata Yayoi

  Nodai Research Institute Tokyo University Of Agriculture

関連論文

• Activation of Mouse Oocytes by Transferring a Nucleus from Fertilized Embryos
• DNA Synthesis in Mouse 1-Cell Embryos Containing Transferred Nuclei
• Production of Live Young by Serial Nuclear Transfer with Mitotic Stages of Donor Nuclei in Mice
• Thymocyte Transfer to Enucleated Oocytes in the Mouse

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