Formation of Ovary-Like Multitissue Spheroids Composed of Isolated Rat Follicles <I>In Vitro</I>

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概要

• 論文の詳細を見る

• Intact preantral follicles were isolated from immature rat ovaries by enzymatic dissociation and cultured in Dulbeccos modified Eagles medium (DMEM) supplemented with 10% fetal calf serum. The globular shape of the follicles was maintained during culture for 96 h on agar-coated plates. When the follicles were placed near the center of the plates, follicles assembled, being in close contact with each other 24 h after culture and after 48 h large spheroidal tissues had formed. Histological examination revealed that these tissues possessed many intact follicles with preserved oocytes and many differentiated cells filled the gaps between follicles. The layer resembled squamous epithelium covered this spheroidal tissue. Culture in serum-free DMEM resulted in follicle assemblage but not formation of ovary-like multitissue spheroids (ovarioids). On the other hand, fetal calf serum induced ovarioid formation in a concentration-dependent manner as did the fraction containing the 30-100-kDa protein from fetal calf serum. Protein and RNA synthesis inhibitors inhibited ovarioid formation. The formation of ovarioids from isolated ovarian follicles may prove to be a useful model to study the ovarian cell-cell or tissue-tissue attachment and the cell differentiation <I>in vitro</I>.

• 日本繁殖生物学会の論文

著者

• MURAKAMI Nobuo

  Noguchi Thyroid Clinic and Hospital Foundation

• MURAKAMI Takayuki

  Department of Veterinary Medicine, Faculty of Agriculture, University of Miyazaki

• MURAKAMI Noboru

  Department of Dermatology, St. Marianna University School of Medicine

• Murakami Takayuki

  Department Of Urology Yokohama City University Graduate School Of Medicine

• Murakami Noboru

  Department Of Endocrinology Kishiwada City Hospital

• KURODA Haruto

  Department of Veterinary Physiology, Faculty of Agriculture, Miyazaki University

• Kuroda Haruto

  Department Of Veterinary Physiology And Faculty Of Agriculture Miyazaki University

• AKAGUMA Osamu

  Departments of Veterinary Physiology and vAnatomy, Faculty of Agriculture, University of Miyazaki

• SUGITA Kouei

  Departments of Veterinary Physiology and vAnatomy, Faculty of Agriculture, University of Miyazaki

• TANIGUCHI Miki

  Departments of Veterinary Physiology and vAnatomy, Faculty of Agriculture, University of Miyazaki

• ETOH Teiichi

  Departments of Veterinary Physiology and vAnatomy, Faculty of Agriculture, University of Miyazaki

• Murakami Noboru

  Department Of Dermatology St. Marianna University School Of Medicine

• KURODA Haruto

  Departments of Veterinary Physiology and vAnatomy, Faculty of Agriculture, University of Miyazaki

• MURAKAMI Noboru

  Departments of Veterinary Physiology and vAnatomy, Faculty of Agriculture, University of Miyazaki

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