Application of DNA Fingerprinting in Evaluation of Pedigree in Dogs
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概要
- 論文の詳細を見る
The reliability of DNA fingerprinting in evaluating the parent/offspring relationship (pedigree) of 5 related hybrid dogs (parents: 2; offspring: 3) and 2 non-related Beagle breeds was investigated with the Myo probe. DNA was isolated from leucocyte, digested with <I>Hinf</I> I before subjecting to agarose gel electrophoresis and transferred to a nylon membrane eventually. The nylon membrane was hybridized with <SUP>32</SUP>P-labeled Myo probe, and the respective bands were detected after autoradiography. Bands derived from the 3 offspring resembled that of either the father or mother. This clearly indicates that DNA fingerprinting can be effectively employed for pedigree evaluation, and may serve as a positive proof of paternity as well.
- 日本繁殖生物学会の論文
著者
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高木 香
日本大学獣医臨床繁殖学研究室
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Takagi Kaoru
Laboratory Of Veterinary Obstetrics & Gynecology Department Of Veterinary Medicine College Of Bi
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Ishiyama Ikuo
Department Of Elementary Education Kokugakuin Tochigi Jr. College
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YOSHII Tomio
Department of Legal Medicine, Teikyo University, School of Medicine
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Yoshii Tomio
Department Of Legal Medicine Teikyo University School Of Medicine
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TAKAGI Kaoru
epartment of Veterinary Obstetrics and Gynecology, College of Agriculture and Veterinary Medicine
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TSUCHIYA Kiyoshi
epartment of Veterinary Obstetrics and Gynecology, College of Agriculture and Veterinary Medicine
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WADA Youhei
epartment of Veterinary Obstetrics and Gynecology, College of Agriculture and Veterinary Medicine
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TAKEISHI Masatoshi
epartment of Veterinary Obstetrics and Gynecology, College of Agriculture and Veterinary Medicine
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KARIYABU Hosaku
Japan Kennel Club
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YUKAWA Sadao
Japan Kennel Club
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ISHIYAMA Ikuo
Department of Legal Medicine, Teikyo University, School of Medicine
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