Application of Parent/Offspring Pedigree in Thoroughbred Horses with DNA Fingerprint
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概要
- 論文の詳細を見る
Application of DNA fingerprinting in evaluating parent/offspring relationship (pedigree) in Thoroughbred horses was attempted with Myo probe. From blood samples of 22 Thoroughbred horses (4 sires, 9 dams and 9 offsprings), DNA was isolated prior to digestion with the restriction endonuclease, Hinf I. A total of 7-16 (11.5 ± 2.1) bands of the DNA fragments were detected with the use of 32P-labeled Myo probe. Of these, 2 bands of all DNA samples coincided at 2.0 and around 3.8 kb. Among 9 parent/offspring pairs, bands from the offsprings well coincided with those of either the sire or dam, advocating that the application of Myo probe for verifying the parent/offspring relationship by the DNA fingerprinting method was possible. Furthermore, to enhance the reliability of parent/offspring verification in Thoroughbred horses by the DNA fingerprinting method, use of numerous probes and restriction endonuclease is indispensable.
- 日本繁殖生物学会の論文
著者
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Takagi K
Nihon Univ. Kanagawa Jpn
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ISHIYAMA Ikuo
Department of Elementary Education, Kokugakuin Tochigi Junior College
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TSUMAGARI Shigehisa
Department of Veterinary Obstetrics and Gynecology, Nihon University
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TAKEISHI Masatoshi
Department of Veterinary Obstetrics and Gynecology, Nihon University
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TAKAGI Kaoru
Second Department of Veterinary Internal Medicine, College of Agriculture and Veterinary Medicine, N
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TSUCHIYA Kiyoshi
Department of Veterinary Obstetrics and Gynecology, College of Agriculture and Veterinary Medicine,
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YOSHII Tomio
Department of Legal Medicine, Teikyo University, School of Medicine
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KITAZIMA Yuzou
Department of Veterinary Obstetrics and Gynecology, College of Agriculture and Veterinary Medicine,
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