STUDIES ON THE FUNCTION OF CELL MEMBRANE 10TH REPORT : EFFECTS OF CCl4 ON THE MARKER ENZYME ACTIVITIES AND FINE STRUCTURES OF RAT LIVER PLASMA MEMBRANES AND MICROSOMES IN VITRO

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Direct action of CCl4 on rat liver plasma membranes and microsomes was investigated biochemically and morphologically in vitro. Plasma membranes or microsomes were pre-incubated with CCl4, then subjected to enzyme assays and electron microscopic observations. In plasma membranes, Mg++-ATPase lost its activity remarkably corresponding to the amount of CCl4, whereas (Na+-K+)-ATPase remained unaffected. 5-nucleotidase and leucine aminopeptidase activities decreased to some extent but only with a large amount of CCl4. NADH-cytochrome c reductase activity also decreased, but not NADH-ferricyanide dehydrogenase activity. Some alterations of unit membrane structure and a complete disappearance of hexagonal patterns were observed electron microscopically in CCl4-pretreated plasma membranes. In microsomes, a marked loss of glucose-6-phosphatase activity was detected. NADH- and NADPH-cytochrome c reductase activities also decreased to some extent, whereas NADH-ferricyanide dehydrogenase activity remained unchanged. Some of these results show similar tendencies to those observed with the in vivo administration of CCl4. Thus, alterations of plasma membranes in vivo may indeed be partly due to a direct action of CCl4.
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