超微小電極法による気管線毛上皮の電気生理学的研究

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Although accumulated studies of the ciliated cells have been made, the initiation and regulation of ciliary beat, and their metachronal coordination remains unknown. The bioelectrical activity at different depths of the rabbit tracheal wall was registered by capillary microelectrode under optimal physiological condition during in vitro experiments. And the influence on the electric potential of the ciliated cell layer and smooth muscle in sodium deficient medium was also investigated. Three characteristic layers of the tracheal wall could be electrophysiologically identified. Espe cially, as the electrode was moved down at a depth of 10-20p, the bioelectric potential fluctuations showing DC-level of -20 to -40mV, with an amplitude of 100-300pV and a frequency varied from 15 to 20Hz were recorded. In consideration of the histological structure of the respiratory mucosa and the frequency of the ordinary ciliary beat rate, it was speculated that these rhythmic oscillations might be ascribed to the intracellular recording of the ciliated cell or to mechanical fluctuation of the microelectrode due to the ciliary movement. However, no remakable changes were seen in the oscillatory potentials when sodium deficient solution was applied to the tissue. Therefore, it is not fully clear from the results whether one action potential corresponds to one ciliary beat.Scanning electron micrographs of the ciliated epithelia which were taken with the microelectrode penetrated into the specimen after measurement of electric activity were useful to clarify the relation between microelectrode and surface architecture of the cell.
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超微小電極法による気管線毛上皮の電気生理学的研究

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