Radioimmunodetection of a local recurrence of rectal carcinoma by 131I-labeled anticarcinoembryonic antigen monoclonal antibody antiCEA 102.

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Radioimmunodetection using monoclonal antibody to tumor-associated antigen has the potential for the differential diagnosis between malignant and benign tissues. Since carcinoembryonic antigen (CEA) is tumor-associated in colorectal cancer, we produced monoclonal antibody antiCEA102 by immunizing Balb/c mouse with purified CEA. The serological specificity of antiCEA102 was analysed by immunoperoxidase method. AntiCEA102 reacted with normal epitherium of fetal colon and almost all of the cancer of the colorectum. AntiCEA102 also reacted with 3/5 gastric cancers, whereas the other cancers or normal tissues tested, including melanoma, astrocytoma and fibrous tissues, were negative. Therefore, antiCEA102 is a good tool to detect the metastatic sites of the colorectal cancer. Abdominoperineal resection was performed 64 year old female. Twenty-nine month later, serumCEA level rose up to 23ng/ml. Chest X-P, CT showed no metastatic tumor in the chest and liver. Therefore, local recurrence was suspected. 0.9mg of antiCEA102 was labeled with 10mCi of <SUP>131</SUP>I by chloramin T method. The patient was given injection of 0.8mCi of the labeled antiCEA102, and an image was obtained close to the bladder seventy-two hours later, which coincided with the mass detected by the pelvic CT.
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