An imunohistological study with formalin-fixed paraffin-embecded tissue sections on malignant colorectal lymphomas using anti-B cell(MB-1) and anti-T cell(MT-1) monoclonal antibodies.

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Identification of the lymphoid cells in primary malignant lymphoma of the gastrointestinal tract is very important, and retrospective studies on past cases are also significant. For this purpose, however, frozen sections of specimens have been commonly used. Therefore, a method using is formalin-fixed, paraffin-embedded specimens is desirable.<BR>In order to analyze three cases of primary malignant lymphoma of the colon and rectum, we attempted to identify the tumor cells on formalin-fixed sections using the monoclonal antibodies, anti-B cell (MB-1), anti-T cell (MT-1), and anti-LeuM 1 (leu M-1). The tumor cells were distinctively stained.<BR>Two cases were B cell malignant lymphoma, and one case was T cell maligrant lymphoma. In one of the cases of B cell malignant lymphoma, the form of the tumor cells changed during observation period, but by this method, it was suggested that the changes involved the same cell type.<BR>It was concluded that this method is technically simple. for identification of the tumor cells derived from malignant lymphoma and that it is useful for retrospective studies.
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