Adenosine 3':5'-Cyclic Monophosphate Inhibits in Vitro Angiogenesis Induced by Endothelial Cell Growth Factor.

スポンサーリンク

概要

• 論文の詳細を見る

• The formation of new blood capillaries (angiogenesis) occurs in response to angiogenic factors released by either normal or tumoral cells. In the present study, we cultured human umbilical vein endothelial cells (HUVEC) on collagen gels and aimed to clarify the effects of cyclic nucleotides on angiogenesis induced by endothelial cell growth factor (ECGF). HUVEC invaded the underlying collagen matrix and formed tube-like structures when ECGF was added. ECGF (9.4 to 75μg/ml) induced angiogenesis in a concentration-dependent manner; the effect reached a plateau at 75μg/ml. Cyclic AMP (10-3M), dibutyryl cyclic AMP (10-3M), 8-bromo cyclic AMP (10-5M) and Sp-cAMPS (10-3M), a stimulator of cyclic AMP-dependent protein kinase, each significantly inhibited ECGF-induced angiogenesis by 64.2, 86.1, 46.5, 74.7%, respectively. Forskolin and cholera toxin, which are activators of adenylate cyclase, did not inhibit ECGF-induced angiogenesis. Dibutyryl cyclic GMP (10-4, 10-3M) also did not affect the formation of capillary-like tubes induced by ECGF. In conclusion, cyclic AMP, but not cyclic GMP, inhibits angiogenesis in vitro. This antiangiogenic activity may be applicable to the treatment of such conditions as solid tumors, diabetic retinopathy and rheumatoid arthritis in which the suppression of angiogenesis is important.

• International Heart Journal刊行会の論文

著者

• Furui Hirohiko

  First Department of Internal Medicine, Nagoya University School of Medicine

• Saito Hidehiko

  First Department Of Internal Medicine Nagoya University School Of Medicine

• Suzuki Toshikazu

  First Department Of Internal Medicine Nagoya University School Of Medicine

• Yamauchi Kazunobu

  First Department Of Internal Medicine Nagoya University School Of Medicine

• FURUMICHI Takeo

  First Department of Internal Medicine, Nagoya University School of Medicine

• YAMADA Yoshiji

  First Department of Internal Medicine, Nagoya University School of Medicine

関連論文

• Structural Organization and Promoter Activity of the Human Ryudocan Gene
• Constitutively Active STAT5A and STAT5B In Vitro and In Vivo: Mutation of STAT5 Is Not a Frequent Cause of Leukemogenesis
• Infectious complications during remission induction therapy in 577 patients with acute myeloid leukemia in the Japan Adult Leukemia Study Group studies between 1987 and 1991
• In vivo Effects of a Histone Deacetylase Inhibitor, FK228, on Human Acute Promyelocytic Leukemia in NOD/Shi-scid/scid Mice
• Apoptotic Cytotoxic Effects of a Histone Deacetylase Inhibitor, FK228, on Malignant Lymphoid Cells
• ABNORMAL POSTEXERCISE SYSTOLIC BLOOD PRESSURE RESPONSE IS A GOOD INDICATOR OF IMPAIRED LEFT VENTRICULAR FILLING DURING SUPINE CYCLE ERGOMETER EXERCISE IN PATIENTS WITH CORONARY ARTERY DISEASE
• -385- A STUDY ON BLOOD COAGULABILITY AND FIBRINOLYTIC ACTIVITY BEFORE AND AFTER REHABILITATION IN PATIENTS WITH ACUTE MYOCARDIAL INFARCTION
• -384- EFFECTS OF TREADMILL EXERCISE ON T-RA, PROTEIN C, FRA, EPBβ-15-42 AND D-DIMER IN PATIENTS WITH ISCHEMIC HEART DISEASE AND NORMAL SUBJECTS
• Clinical Trial to Investigate the Pharmacokinetics, Pharmacodynamics, Safety, and Efficacy of Recombinant Factor VIIa in Japanese Patients With Hemophilia With Inhibitors
• Histopathologic Evaluation of Coronary Artery Thrombi Obtained by Directional Coronary Atherectomy in Patients With Restenosis-Induced Unstable Angina Pectoris
• AJMALINE-INDUCED NORMALIZATION OF ATRIOVENTRICULAR CONDUCTION IN PATIENTS WITH WPW ECG : PROCEEDINGS OF THE 37TH ANNUAL MEETING OF THE JAPANESE CIRCULATION SOCIETY
• Molecular Cloning, Genomic Organization, Promoter Activity, and Tissue-Specific Expression of the Mouse Ryudocan Gene^1
• Analysis for heterozygosity of protein S mRNA: application to genetic screening and family studies in hereditary protein S deficiency
• Correlation Between the Exercise-Induced Increase in Left Ventricular Filling Pressure and the Extent of Ischemic or Infarcted Myocardium
• EFFECTS OF TREADMILL AND HANDGRIP EXERCISE ON BLOOD COAGULABILITY AND PLATELET FUNCTION IN PATIENTS WITH ISCHEMIC HEART DISEASE : Excercise Test : IV : 48 Annual Scientific Meeting, Japanese Circulation Society
• Posttreatment Nadir M-Protein Level Is a Stronger Discriminator of Survival Following Plateau Attainment Than Is Percent Reduction in M-Protein in Patients With IgG Myeloma
• Clonality analysis of granulocytes and T lymphocytes in healthy females by the PCR-based HUMARA method
• Serum thrombopoietin level after allogeneic bone marrow transplantation: possible correlations with platelet recovery, acute graft-versus-host disease and hepatic veno-occlusive disease
• EFFECTS ON ISOTONIC AND ISOMETRIC EXERCISES ON PLATELET FUNCTION AND BLOOD COAGULABILITY IN HEALTHY MEN : PROCEEDINGS OF THE 47th ANNUAL SCIENTIFIC MEETING OF THE JAPANESE CIRCULATION SOCIETY : Exercise Test
• 6)RESPIRATORY CHANGES IN NORMAL FRANK VECTORCARDIOGRAPHIC P LOOP : THE II CONFERENCE ON VECTORCARDIOGRAPHY
• 58(4) 238-247 USE OF A THERMODILUTION RENAL VEIN CATHETER TO MEASURE RENAL BLOOD FLOW IN PATIENTS WITH ESSENTIAL HYPERTENSION
• Protein Expression and Constitutive Phosphorylation of Hematopoietic Transcription Factors PU.1 and C/EBPβ in Acute Myeloid Leukemia Blasts
• Identification of six novel MYH9 mutations and genotype-phenotype relationships in autosomal dominant macrothrombocytopenia with leukocyte inclusions
• Identification of plasma antibody epitopes and gene abnormalities in Japanese hemophilia a patients with factor VIII inhibitor
• Infrequent Mutation of the hBUB1 and hBUBR1 Genes in Human Lung Cancer
• Effect of Gene Transfer of Tumor Necrosis Factor Receptors into Human Lung Carcinoma Cell Line
• Effects of Various Mutations in the Neurophysin/Glycopeptide Portion of the Vasopressin Gene on Vasopressin Expression in Vitro
• Long-term Results of a Multicenter Randomized, Comparative Trial of Modified CHOP versus THP-COP versus THP-COPE Regimens in Elderly Patients with Non-Hodgkin's Lymphoma
• Immune response of post-transplant peripheral lymphocytes against the patient pre-B cell line, NAGL-1
• Acute myeloid leukemia in the elderly : 159 Nagoya case studies
• Recovery of Normal Hematopoiesis after Severe Bone Marrow Aplasia Induced by Interferon-α in a Patient with Chronic Myelogenous Leukemia
• Molecular evaluation of bone marrow involvement in peripheral T-cell lymphoma with a PCR-mediated RNase protection assay
• Homozygous type IIC von Willebrand's disease
• Efficacy of a New Formulation of Lenograstim (Recombinant Glycosylated Human Granulocyte Colony-Stimulating Factor) Containing Gelatin for the Treatment of Neutropenia After Consolidation Chemotherapy in Patients With Acute Myeloid Leukemia
• No Significant Association Between HA-1 Incompatibility and Incidence of Acute Graft-Versus-Host Disease After HLA-Identical Sibling Bone Marrow Transplantation in Japanese Patients
• CD4- and CD56-positive T-cell line MTA, established from natural killer-like T-cell leukemia/lymphoma
• MICROCOMPUTER-ASSISTED VECTORCARDIOGRAPHIC ANALYSIS : PROCEEDINGS OF THE 47th ANNUAL SCIENTIFIC MEETING OF THE JAPANESE CIRCULATION SOCIETY : Vectorcardiography Mapping
• THE CLINICAL SIGNIFICANCE OF HEMOGLOBIN OXYGEN DISSOCIATION CURVE IN PATIENTS WITH CONGESTIVE HEART FAILURE : PROCEEDINGS OF THE 47th ANNUAL SCIENTIFIC MEETING OF THE JAPANESE CIRCULATION SOCIETY : Heart Failure
• VCG DIFFERENTIAL DIAGNOSIS BETWEEN PREMATURE VENTRICULAR CONTRACTION AND ABERRANT VENTRICULAR CONDUCTION : Vectorcardiography : 46th Annual Scientific Meeting, Japanese Circulation Society
• EFFECTS OF TREADMILL EXERCISE ON HEMOGLOBIN AFFINITY FOR OXYGEN IN NORMAL SUBJECTS : Exercise Test : 46th Annual Scientific Meeting, Japanese Circulation Society
• IgD monoclonal gammopathy of undetermined significance
• Sex and Age Differences of Normal P Loops in the Frank Lead Vectorcardiogram
• -P536- INFLUENCE OF VARIOUS ANTI-HYPERTENSIVE DRUGS ON PROFILES IN 24-HOUR BLOOD PRESSURE AND HEART RATE VARIATION(PROCEEDINGS OF THE 59th ANNUAL SCIENTIFIC MEETING OF THE JAPANESE CIRCULATION SOCIETY)
• In Vitro Effects of Recombinant Human IL-11 on Human Malignant Lymphoma Cells
• Hemoglobin Pitie-Salpetriere [β34 (B16) Val→Phe] Showing Erythrocytosis and Mild Hemolysis in a Japanese Man
• Acute Graft-Versus-Host Disease of the Large Intestine after Bone Marrow Transplantation
• A pathological role of increased expression of plasminogen activator inhibitor-1 in human or animal disorders
• Effects of treadmill exercise on platelet function, blood coagulability and fibrinolytic activity in patients with atrial fibrillation.
• Adenosine 3':5'-Cyclic Monophosphate Inhibits in Vitro Angiogenesis Induced by Endothelial Cell Growth Factor.
• Effects of treadmill exercise on platelet functions and blood coagulating activities in healthy men.
• Plasma .BETA.-Thromboglobulin and platelet factor 4 concentrations in patients with atrial fibrillation.
• Plasma t-PA/PAI-1 Complex and Blood Coagulability in Patients with Coronary Artery Disease.
• Evaluation of myocardial involvement in Duchenne's progressive muscular dystrophy with thallium-201 myocardial perfusion imaging.
• Inhibitory effects of endothelial cells and calcium channel blockers on platelet aggregation.

もっと見る 閉じる

スポンサーリンク