Live Detection of the Firefly Luciferase Gene Expression by Bioluminescence in Incubating Chicken Embryos

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概要

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• By using in ovo electroporation, the firefly luciferase gene was transfected to living chicken embryos at 2 days of incubation. At 48hrs after transfection, expression of the firefly luciferase gene was monitored in ovo by using a single photon imaging counter. Strong bioluminescence detected from the incubating embryo suggests that in ovo electroporation would provide a useful and efficient nonviral means of foreign gene transfection to somatic cells of living chicken embryos.

• 社団法人 日本畜産学会の論文

著者

• Okumura Jun-ichi

  Laboratory Of Animal Nutrition Graduate School Of Bioagricultural Sciences Nagoya University

• MURAMATSU Tatsuo

  Laboratory of Animal Nutrition, School of Agricultural Sciences, Nagoya University

• OKUMURA Jun-ichi

  Laboratory of Animal Nutrition, School of Agricultural Sciences, Nagoya University

• MIZUTANI Yoshimoto

  Laboratory of Animal Nutrition, School of Agricultural Sciences, Nagoya University

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• Live Detection of the Firefly Luciferase Gene Expression by Bioluminescence in Incubating Chicken Embryos

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