イオン交換クロマトグラフィ-によるカテコ-ルアミンの抽出と高速液体クロマトグラフィ-による測定〔英文〕

概要

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Catecholamine was extracted from various tissues of mice by Amberlite CG50 column chromatography with high recoveries (more than 90%). Epinephrine, norepinephrine and dopamine were separated by high-pressure liquid chromatography with a reversed-phase column and an electrochemical detector and simultaneously measured by the reading of peak heights on the high-pressure liquid chromatographic recorder. Correlation between the peak height and the amount of standards applied to the column was high (r=0.99). Inter- and intra-assay coefficients of variation were less than 2.6 and 3.0%, respectively. The detection limit (signal/noise>2) was 75 fmol for norepinephrine and 100 fmol for epinephrine and dopamine. When tissue samples were applied onto alumina columns, some unidentified substances were extracted with catecholamines. However, these unidentified substances were removed by selective adsorption to CG50 columns and, even if present, did not interfere with measurement of catecholamine. These results suggest that this method can be generally applied to the measurement of endogenous catecholamines